Characterisation of a novel laminarinase from Microbulbifer sp. ALW1 and the antioxidant activity of its hydrolysates

Laminarin and its derived oligosaccharides have diverse bioactivities. The β-1,3-glucanase in marine bacteria can be employed as a tool to digest laminarin in the cell wall of brown algae. Here, we cloned, expressed and characterised a β-1,3-glucanase (laminarinase), MaLamNA, from the previously characterised marine bacterium Microbulbifer sp. ALW1, phylogenetically distinct from the glycoside hydrolase families of characterised laminarinases. The recombinant laminarinase was heterologously expressed and purified from Pichia pastoris GS115 cells with a molecular mass of 57.3 kDa. MaLamNA exerted its hydrolytic activity specifically against laminarin, with the highest activity at 45 °C and pH 4.5–5.5, respectively, and demonstrated high stability against extreme acidic and alkaline pH exposure. The addition of reducing agent dithiothreitol could significantly enhance the activity of MaLamNA. The hydrolytic products of laminarin by MaLamNA exhibited more effective antioxidant activities than the undigested laminarin. These characteristics of MaLamNA provide clues to its industrial application for laminarin bioresource development.     

Towards performance improved anion exchange membrane: Cross-linking with multi-cations oligomer modified graphene oxide

Cross-linking structure has been proven to be an effective approach to address the balance issue between ionic conductivity, dimensional stability and other properties of anion exchange membranes (AEMs). Here, a novel multi-cationic oligomer was synthesized from 1,4-diazabicyclo [2,2,2]octane and 1,6-dibromohexane, and subsequently used to prepare multi-cationic oligomer brushes-decorated graphene oxide (QBGO). The obtained QBGO was employed as the cross-linker to form cross-linked poly (arylene ether sulfone) (QPAES) AEMs by end-cap tertiary amine coupling reaction. Benefiting from the introduction of the multi-cations and flexible long-chain cross-linker structure, the cross-linked QPAES/QBGO membranes formed hydrophilic/hydrophobic phase-separation microstructures and well-defined ionic channels which are responsible for water uptake and ion transfer. As a result, the cross-linked QPAES/QBGO-2.0 membrane exhibited 1.90-fold higher ionic conductivity and better chemical stability than the control QPAES membrane. The QPAES/QBGO-2.0 membrane displayed a higher power density of 75.7 mW cm^?2 than that of the control QPAES membrane (53.1 mW cm^?2) in a H₂/O₂ fuel cell test. In a word, we propose that this novel design strategy holds broad prospects for the design of new polymer electrolyte membrane materials.     

水泥工厂联合储库智能抓斗行车系统

目前，水泥生产线的原燃料储存及均化，大部分采用的都是配有堆取料机的预均化堆场。其优点是对物料的预均化效果好，但不足之处如占地面积较大，取黏湿物料时效率较低，堆取料机错位时需要人工参与，对实现二代水泥技术对劳动定员的高要求不利。 近年来，随着电控、传感及相关智能技术（如：料位3D模拟、智能生产控制方案）的发展，采用智能抓斗的全自动无人值守联合储库作为一种技术方案正在逐步实现。     

四川盆地震旦系灯影组丘滩体发育分布及对储层的控制

四川盆地震旦系灯影组储层主要发育于微生物丘滩体中，但不同成因类型丘滩体对储层的影响尚不明确。通过钻井岩心和野外露头观测、岩石薄片分析和物性测试等手段，将丘滩体划分为丘基、丘核、丘翼和丘坪4种微相，并根据微相的组合划分出台缘并进型丘滩体、台缘追补型丘滩体和台内饥饿型丘滩体3种丘滩体类型。台缘并进型丘滩体的生长速率快，与可容空间的增加速率相同步，其微相组合以"丘基+丘核"垂向加积为主，在海平面升降过程中易暴露于大气淡水环境，造成海水胶结作用弱、淡水溶蚀作用强，形成以格架溶蚀孔洞为主的储集空间，储层物性好，储集性能最优。台缘追补型丘滩体的生长速率次之，其早期的生长速率滞后于可容空间增长速率但后期逐渐同步，呈现出"丘基+丘核+丘翼/丘坪"侧向加积的微相组合；由于暴露于大气淡水环境的几率减小，其海水胶结作用强，淡水溶蚀作用减弱，储集性能稍差。台内饥饿型丘滩体的生长速率最慢，严重滞后于可容空间的增长速率，呈"云坪（丘基）+丘核+丘坪"微相组合，难以暴露于大气淡水环境，淡水溶蚀作用最弱，储集性能也最差。台缘带古地貌对丘滩体的生长速率影响较大，可控制丘滩体的沉积样式。陡坡台缘带有利于台缘并进型丘滩体发育，缓坡台缘带主要发育台缘追补型丘滩体，台内高地中发育台内饥饿型丘滩体。胡家坝-阆中-安岳地区一带的震旦系灯影组四段发育于典型的陡坡台缘带，是储层发育的最有利相带。     

Lead-free sodium niobate nanowires with strong piezo-catalysis for dye wastewater degradation

In this work, the hydrothermally-synthesized sodium niobate nanowires were used to decompose Rhodamine B dye solution through the piezo-catalytic effect. With the sodium niobate catalyst, a high piezo-catalytic degradation ratio of ～80% was achieved under the excitation of vibration for the Rhodamine B dye solution (～5?mg/l). These active species in the catalytic process, hydroxyl radicals and superoxide radicals with the strong oxidation ability, were also observed, which confirmed the key role of piezoelectric effect for piezo-catalysis. The piezo-catalysis of sodium niobate nanowires provides a high-efficiency and reusable tool in application in depredating the dye wastewater.     

Tuning effect of silicon substitution on magnetic and high frequency electromagnetic properties of R2Fe17 and their composites

In this work, we report the tuning effect of the Si substitution on the magnetic and high frequency electromagnetic properties of R₂Fe₁₇ compounds and their paraffin composites. It is found that the introduction of Si can remarkably improve the magnetic and electromagnetic properties of the R₂Fe₁₇ compounds, making the R₂Fe_17–xSi_x-paraffin composites excellent microwave absorption materials (MAMs). By introducing the Si element, their saturation magnetizations decrease slightly, while much higher Curie temperatures are obtained. Furthermore, better impedance match is reached due to the decrease of the high-frequency permittivity ε′ by about 40%–50%, which finally enhances the performance of the microwave absorption. The peak frequency (f_RL) of the reflection loss (RL) curve moves toward high frequency domain and the qualified bandwidth (QB, RL ≤ ?10 dB) increases remarkably. The maximum QB of 3.3 GHz (12.0–15.3 GHz) is obtained for the Sm_1.5Y_0.5Fe₁₅Si₂-paraffin composite (d = 1.0 mm) and the maximum RL of ?53.6 dB is achieved for Nd₂Fe₁₅Si₂-paraffin composite (d = 2.2 mm), both surpassing most of the reported MAMs. Additionally, a distinguished dielectric microwave absorption peak is observed, which further increases the QB in these composites.     

基于多尺度双线性卷积神经网络的多角度下车型精细识别

针对多角度下车辆出现一定的尺度变化和形变导致很难被准确识别的问题，提出基于多尺度双线性卷积神经网络（MS-B-CNN）的车型精细识别模型。首先，对双线性卷积神经网络（B-CNN）算法进行改进，提出MS-B-CNN算法对不同卷积层的特征进行了多尺度融合，以提高特征表达能力；此外，还采用基于中心损失函数与Softmax损失函数联合学习的策略，在Softmax损失函数基础上分别对训练集每个类别在特征空间维护一个类中心，在训练过程中新增加样本时，网络会约束样本的分类中心距离，以提高多角度情况下的车型识别的能力。实验结果显示，该车型识别模型在CompCars数据集上的正确率达到了93.63%，验证了模型在多角度情况下的准确性和鲁棒性。     

盐酸溶液中Fe3+的水解聚合边界条件

为了模拟Pu⁴⁺水解聚合行为，采用动态光散射法研究了盐酸溶液中Fe³⁺水解聚合边界条件，并采用热力学软件HSC Chemistry 6.0模拟计算了水解聚合过程中一些热力学函数值的变化。结果表明：在Fe³⁺发生水解聚合反应时，溶液中的c₀(H⁺)、c(Fe³⁺)和温度之间存在一定的关联性，提高Fe³⁺浓度和升高温度会促进Fe³⁺水解聚合反应；聚合胶体粒径随着Fe³⁺浓度和温度升高而分布越广，且测得中值粒径在100~1 000 nm。